解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Activating the protein-tyrosine kinase activity of v-Src rapidly induced expression of the two 'primary response' genes, TIS10 and Egr-1, in Balb/c 3T3 cells. Depleting cells of protein kinase C (PKC) by prolonged exposure to 12-O-tetradecanoylphorbol 13-acetate (TPA), blocked v-Src-induced TIS10 expression, but had n...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Clinical and experimental evidence is consistent with a key role for transforming human papilloma viruses (HPVs) in the aetiology of anogenital carcinoma. Cervical carcinoma does, however, occasionally occur in the absence of HPV sequences (Riou et al., 1990). We have used a direct cDNA/PCR sequencing protocol to anal...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Transmembrane tyrosine kinases are involved in the control of cell growth and differentiation by extracellular signals. To enable identification of new receptor tyrosine kinases we developed a method that selectively amplifies segments of receptor genes. The method is based on a combination of polymerase chain reactio...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::MCF2/DBL is an X-linked proto-oncogene encoding a protein with a yet undetermined function. It can be activated in vitro by loss of 5' sequences in NIH3T3 bioassays; in vivo, deletion of the gene has been found in some hemophilia B patients. PCR analysis of its expression in mouse tissues shows a restriction to the go...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::We reported previously that the v-rel protein (p59v-rel) exists in a high molecular weight complex with at least four other proteins in the cytoplasm of v-rel-transformed chicken pre-B lymphoid cells (Simek, S. & Rice, N.R., J. Virol., 62, 4730-4736, 1989). One of these proteins is the chicken c-rel protein, but the i...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Genomic clones encompassing the human ETS1 gene were isolated and utilized to define its molecular organization. This gene consists of eight exons spanning over 60 kb. The 5' end of the human ETS1 gene was subcloned and characterized. S1 nuclease, primer extension and RNAase protection analyses of human mRNAs showed m...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We have characterized a novel chicken c-myb exon whose sequences are specifically expressed in thymic cells. In situ hybridization experiments indicate that this thymus-specific coding exon is localized on a small chromosome, distinct from the large acrocentric chromosome 3 on which we recently mapped the bulk of 15 e...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We report the detailed molecular characterization of a human parathyroid adenoma with a clonal parathyroid hormone gene rearrangement. This rearrangement is similar to one we characterized recently in an independent adenoma. In these two, plus a third partially characterized adenoma, one allele of the PTH gene, on 11p...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::The mouse pancreatic amylase Amy-2.2 gene was fused to the structural gene for SV40 T antigen, and 51 independent transgenic founder mice carrying the fusion gene were generated. The majority of the founders and 100% of their offspring in the derived transgenic lines developed pancreatic acinar cell carcinomas and sto...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The E3 region of adenovirus induces down-regulation of epidermal growth factor receptor (EGFR) through endocytosis. Here we report that an EGFR-related protein, the HER-2/c-erbB-2 gene product, p185, is also down-regulated by adenovirus, but via a different mechanism. We found that the adenovirus E1a gene is responsib...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The v-myb oncogene of the avian myeloblastosis virus encodes a nuclear protein, p48v-myb, which binds to DNA in a sequence-specific manner. We have used wild type and mutant forms of this protein expressed in E. coli to study the protein and DNA determinants for sequence-specific DNA-binding. We have shown that only t...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Expression of the c-myc gene is often elevated in human colorectal tumors, but reported amplification of the locus is rare. Here we demonstrate that modest amplification of c-myc is frequently found in aggressive subtypes of colorectal cancer. Careful quantitation of c-myc copy number has shown amplification in 53.8% ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The c-fos proto-oncogene is found to be overexpressed at least 30-fold in SMS-SB, a pre-B leukemic cell line compared to other cell types. No gross alteration of the c-fos gene structure in SMS-SB cells can be detected by karyotypic or Southern analyses. C-fos in SMS-SB cells can still be induced by serum, TPA and the...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::We have characterized a chromosomal translocation in a cell line (SU-DUL5) established from a patient with lymphoblastic lymphoma in which the c-myc gene on chromosome 8 was juxtaposed to a t(14;18). Cytogenetic analysis of this cell line showed 14q+, 18q-, and 8p+q+ marker chromosomes in the absence of t(14;18). Geno...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Drug-resistant clones selected from FR3T3 rat cells after transfer of neo-BPV1 (Bovine Papillomavirus Type 1) DNA constructs became phenotypically transformed (focal transformation, growth in suspension and tumor formation) soon after selection (approximately 5 generations in culture). A frameshift mutation in ORF E5 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Permanent alterations of the epithelial differentiation pattern were investigated after infection of HH-16 cl. 4 adenocarcinoma cells with Moloney murine sarcoma virus (MoMuSV). Transformed cell clones with fibroblastoid morphology were isolated and compared with clones of unchanged epithelioid phenotype. Southern blo...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::We have recently identified and cloned the gene for a cytosolic polypeptide, designated oncoprotein 18 (Op18), which is expressed in acute lymphocytic leukemia and some solid tumors including neuroblastoma. Op18 is phosphorylated upon treatment of lymphoid cells with phorbol myristate acetate. We have proposed that un...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::A transgenic mouse family expressing the middle T antigen of polyoma virus under control of the immunoglobulin heavy chain (IgE) enhancer showed the frequent occurrence of carcinomas in various organs, predominantly in females. Most frequently affected were the salivary and thyroid glands, but mammary tumours, liver h...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::In previous studies examining the role of pp60c-src in cellular proliferation, we demonstrated that overexpression of wild type (wt) but not mutated c-src in C3H10T1/2 murine embryo fibroblasts resulted in a 2 to 5 fold enhancement of DNA synthesis in response to epidermal growth factor (EGF). Using phosphotyrosine an...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::The human ETS2 gene is a member of the ets gene family (ETS1, ETS2, ERG, ELK1 and ELK2) with amino acid similarity to the v-ets oncogene of the avian leukemia virus, E26. The ETS2 gene is composed of 10 exons, nine of which contribute to the open reading frame encoding 469 amino acids. The ETS2 gene directs the synthe...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::The p53 gene has been implicated as a tumor suppressor gene with mutations found in common human cancers. We examined 51 early stage, primary, resected non-small cell lung cancer specimens using an RNAase protection assay and cDNA sequencing. Mutations changing the p53 coding sequence were found in 23/51 (45%) tumor s...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Using a combination of raf and myc oncogenes co-expressed by the recombinant retrovirus J-2 we have generated and characterized a cell line which very efficiently supports the growth of B-cells and B-cell hybridomas. Murine spleen cells were cultured under in vitro immunization conditions favoring the short term proli...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::A new class of nontransformed revertant cells has been isolated from the ras-transformed cell line DT using cis-4-hydroxy-L-proline (CHP) as a selective agent. The new revertants, CHP 9CJ and CHP CB4, each contain two copies of the v-Ki-ras gene, elevated levels of phosphorylated p21ras protein, and rescuable transfor...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Protein kinases are known to undergo phosphorylation to regulate their activity. To determine whether the protein kinase activity of p37v-mos was similarly regulated, we investigated the influence of two well known protein kinases, namely protein kinase C and protein kinase A, on the activity of p37v-mos in vivo. NIH3...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::We have established conditions for the immortalization of human fibroblasts by the large T antigen of the rodent virus polyoma. This allows the mechanism of immortalization to be studied, without interference by transformation events, in cells with relatively stable chromosomes. Large T antigen could immortalize human...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Treatment of quiescent Balb/3T3 clone A31-1-1 cells with 0.1-0.2 mM H2O2 in the presence of 1 microM insulin induced DNA synthesis 20-24 h later at almost the same level as that in cells treated with 10% serum. Treatment with 0.1-0.2 mM H2O2 alone did not induce DNA synthesis and was not toxic to the cells. Cell cycle...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::The HER-2/neu proto-oncogene is homologous with, but distinct from, the epidermal growth factor receptor. Current evidence indicates that this gene is frequently amplified and/or overexpressed in some human breast and ovarian cancers and that these alterations may be clinically important; however, little is known abou...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Previous reports have demonstrated that E7 is the major transforming gene of HPV-16 and that continued expression of the gene is required to maintain the transformed phenotype of primary baby rat kidney cells transformed by HPV-16 E7 and EJ-ras. To investigate the point of action of E7 in the cell cycle we have utilis...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The products of two cellular proto-oncogenes c-fos and c-jun form a heterodimeric complex that contribute to the DNA-binding activity referred to as AP-1 (activator protein-1). Two domains have been proposed to be required for heterodimer formation and protein-DNA complex formation. The leucine zipper domain mediated ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::Each step in the synthesis of functional transcript by RNA polymerase II provides a level at which gene expression can be regulated. Control over the elongation phase of transcription is a recognized regulatory mechanism in prokaryotes; however, only recently have examples of conditional transcription elongation block...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:
更新日期:1990-06-01 00:00:00
abstract::A series of primary human breast tumors was analysed by immunoblotting with anti-neu antibodies. Overproduction of the neu protein-tyrosine kinase, p185neu, was observed in 23 of 56 malignant tumor samples. 29 of these tumors were also immunoblotted with antiphosphotyrosine antibodies. A single prominent phosphotyrosi...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::A 1.26 kb murine cDNA having 31% homology with human ras and 55% homology with human rho proteins was isolated using an oligonucleotide probe homologous to catalytic subdomain of a tyrosine kinase subfamily. Northern blot analysis indicates that the expression of the murine gene is restricted to the cells of hemopoiet...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::Cell lines derived from cervical neoplasias, as well as cells from normal cervix and neonatal foreskin were examined in an in vitro culture system (raft system) that allows for stratification and differentiation of epithelial cells at an air-liquid interface. Epithelial cells from human foreskin and ectocervix were ob...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Ras genes are activated by point mutations at critical sites of their coding regions. Activated N-ras genes with transforming ability have been detected in patients with myelodysplastic syndromes (MDS), acute myelogenous leukemia (AML) and in human myeloid cell lines. We used polymerase chain reaction (PCR), different...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Clinical and epidemiological data support a role for human papilloma virus (HPV) type 16 in the pathogenesis of cervical carcinoma. The W12 cell line contains HPV16 sequences, predominantly as a high copy number episome, and is immortalised in vitro, but non-tumourigenic. A morphologically distinct sub-line of the par...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Previous studies have shown that increased expression of oncogenes from the myc-family can down-regulate the level of MHC class I antigens in tumor cells. This has suggested a mechanism by which amplification/overexpression of myc-genes may contribute to the malignancy development of certain tumors. Earlier published ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The eph gene encodes a putative receptor tyrosine kinase for an as yet unknown ligand. Some human cancer cells have been found to overexpress eph mRNAs without gene amplification. We show here that NIH3T3 cells acquire tumorigenic ability in nude mice and make colonies in soft agar with a viral LTR (Long Terminal Repe...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The human c-abl oncogene gives rise to different mRNA transcripts which vary primarily in that they possess alternative first exons. In the present study, we present the sequence for the human c-abl 3' untranslated region (3'utr) and show that while human and murine c-abl cDNA sequences are generally homologous, human...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Differentiation inducible leukemic as well as normal myeloid precursors treated with physiological myeloid differentiation inducer have been used to explore the immediate early genetic response of cells to terminal differentiation and growth arrest stimuli. cDNA clones of 12 distinct genes, referred to as MyD genes, w...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::cDNA clones of human fos-related genes fra-1 and fra-2 were isolated by screening human c-DNA libraries with human fos DNA as a probe. We obtained human fra-1 cDNA clones that can code for a protein of 271 amino acid residues with a calculated molecular weight of 29,413 and showed 90% similarity with rat fra-1 protein...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00